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Isolation and Identification of a Highly Efficient Strain of Aniline Degrading Bacterium Q6 and Degradation Characteristics
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School of Environment and Safety Engineering, Central North University, Taiyuan 030051, China

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    Abstract:

    The production and processing of pyrotechnics produce aniline wastewater, which causes a great pollution to the environment. To provide an efficient strain resource for aniline degradation, a strain Q6 with aniline as the only carbon source and energy growth was isolated from the activated sludge in the aerobic aeration tank of the chemical wastewater treatment plant. Through morphological observation, physiological and biochemical characteristics and 16S rDNA gene sequence phylogeny analysis, the strain was identified as Acidovorax sp. The effects of inoculum amount, temperature, pH value and additional carbon and nitrogen sources on aniline degradation by strain Q6 were studied. Meanwhile, the degradation kinetics of aniline at different initial concentrations and the growth kinetics of strain Q6 were fitted. The results showed that under the conditions of temperature 23-37 ℃ and pH 5-8, strain Q6 could degrade aniline efficiently, and ammonium chloride was the best co-metabolizing nitrogen source to promote the degradation of aniline.The optimum conditions of 33 ℃, pH 7 and inoculation amount of 10%, the degradation rate of aniline at different initial concentrations (200-3000 mg·L-1) was above 95.0%, and the degradation process of aniline showed first-order and zero-order kinetics. The growth process of Q6 conformed to the Haldane equation with a maximum specific growth rate μmax of 0.130 h-1, a half-satiation constant Ks of 190 mg·L-1 and the inhibition constant Ki of 8497 mg·L-1. The results show that strain Q6 has unique potential in the treating industrial wastewater containing high concentration of aniline.

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胡锦俊,白红娟,宋雨,等.一株高效降解苯胺菌Q6的分离鉴定及其降解特性[J].含能材料,2022,30(10):1004-1012.
HU Jin-jun, BAI Hong-juan, SONG Yu, et al. Isolation and Identification of a Highly Efficient Strain of Aniline Degrading Bacterium Q6 and Degradation Characteristics[J]. Chinese Journal of Energetic Materials,2022,30(10):1004-1012.

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History
  • Received:June 24,2022
  • Revised:September 12,2022
  • Adopted:September 05,2022
  • Online: September 06,2022
  • Published: October 25,2022