A rapid and accurate high performance liquid chromatographic method was developed for the simultaneous determination of TATB and HMX in explosive formulations. Dimethyl sulfoxide （DMSO） was used as the solvent and ultrasonic assisted to dissolve the sample. The influences of sample weight， dissolve time， mobile phase type， chromatographic column type and detection wavelength on results were investigated. The results show that HMX and TATB were confirmed to separate within 6.5 min by using C18 chromatographic column with mobile phase of acetonitrile and water at 45/55（V/V）. And the HMX and TATB could be extracted completely at the condition of ultrasonic for 1.0 h and standing at room temperature for more than 4.0 h after they were dissolved in 250 mL DMSO. The relative standard deviation of reproducibility （n=8） was 0.55% and 0.99%， respectively. Linear relationships between values of peak area and mass concentration of TATB and HMX were good in the ranges of 0.032-0.056 mg·mL^-1（R²=1.0000） and 0.011-0.016 mg·mL^-1（R²=0.9999）.