In order to improve the detection performance of the fluorescent probe to the peroxide explosives, hydrogen peroxide (H₂O₂) is typically taken as a signature compounds, and a new method for H₂O₂ will facilitate security monitoring of peroxide explosives. In this study, a novel fluorescent probe (4-(4-nitro-benzyl)-boron-dipyrromethene, BD-3) was firstly designed and synthesized by using benzyl as the recognition group and boron-dipyrromethene (BODIPY) as the fluorophore. Meanwhile, the chemical structure of BD-3 was characterized and its detection performance was investigated. The fluorescence of BD-3 itself was very weak with the maximum absorption/emission wavelength of 500/508 nm. When BD-3 was fully reacted with H₂O₂, the fluorescence intensity of BD-3 was increased by approximately 11-fold and observed by the naked eye. The Turn-on fluorescence of BD-3 with response to H₂O₂ was based on the mechanism of photo induced electron transfer (PET). The linear range of BD-3 responding to H₂O₂ was from 1×10^-6 to 1×10^-4 mol·L^-1 with the correlation coefficient of 0.9998, and the detection limit was 0.9 μmol·L^-1. In addition, BD-3 displayed good photo-stability and high selectivity when responded to H₂O₂. In conclusion, this new probe BD-3 had many advantages of good photo-stability, high selectivity and fluorescence enhancement to H₂O₂, and it could meet the requirement of on-site detection of peroxide explosives under complex environmental conditions.