The centimeter-level TNT single crystal was prepared using a solvent evaporation method. Its crystal structure was characterized by XRD and DSC. Results show that the crystal is monoclinic, the biggest crystal face is the (100) plane. The average indentation modulus and hardness of the (100) plane obtained at different indentation load by a nanoindenter are (17.40±0.35) GPa, (0.81±0. 05) GPa, respectively, which is significantly higher than the compression modulus of TNT casting explosive.