A kind of antistatic nitrocellulose (NC) membrane was successfully prepared via the liquid phase mixing complex method using NC as raw materials and selecting carbon series conductive filler to NC. The antistatic performance, mechanical property and microstructure of antistatic NC membrane were characterized by high resistance meter, static sensitivity tester, electronic universal testing machine and field emission scanning electron microscope, respectively. The results show that when 1.0% conductive filler is added, the surface resistivity and volume resistivity of the antistatic NC membrane are 2.2×10⁹ Ω and 7.5×10⁹ Ω·m. respectively. Compared with unmodified NC, the surface resistivity and volume resistivity of the antistatic NC membrane are reduced by 6 orders of magnitude and 3 orders of magnitude, respectively. The electrostatic spark sensitivity of the antistatic NC membrane is 2.31 J. Compared with unmodified NC the electrostatic spark sensitivity of the antistatic NC membrane is increased by 14%, revealing that the antistatic NC membrane has better antistatic performance. The mechanical property parameters of the antistatic NC membrane are improved, showing that the addition of conductive filler does not degrade the mechanical property of NC.The formation of interlaced conductive networks in the products is conduciue to the electrostatic charge dissipate.