In order to find sensing materials used for explosives detecting, fluorescence analysis technique was used to study the fluorescence response of the fluorescence of conjugated compounds with small molecular weight and fluorescent property ethyl 2-(chloromethyl)-4-phenyl-quinoline-3-carboxylate (ECMPQC) to trace nitro-explosives in practice, and fluorescence quenching efficiency with various concentration of nitro-explosives. Electron transfer occurs when ECMPQC with nitro-explosives (such as TNT, HNS and HMX) which are lack of electrons in their molecular structures. As a result, the fluorescence intensity decreases in this system and the fluorescence intensity decreases with the concentration of TNT increasing. ECMPQC has potential application in nitro-explosives detection. The fluorescence property of ECMPQC was investigated in chloroform, acetone and ethanol. Results show that with the increase of solvent polarity, the bathochromic shift of the emission maximum wavelength becomes more remarkable. In ethanol, a new emissive band which is assigned to the formation of the exciplex is appeared in the region of 365~450 nm in fluorescence spectrum between ECMPQC and nitro-explosives.