Submicron/nano-HNS with different morphologies was prepared by prefilming twin-fluid nozzle assisted solvent and non-solvent crystallization process. By controlling of non-solvent temperature,non-solvent pH value and different surfactants,the effect of these factors on morphology of submicron/nano-HNS were analyzed. Results show that the morphology of HNS changes from short plane-like shape to long plane-like shape when non-solvent temperature changes from 25 ℃ to 50 ℃. The morphology comes through the variety of ellipsoid,short plane-like and club-shaped with pH value of non-solvent from acidity to neutral and finally basical,with the particle size distribution in 50-300 nm,50-500 nm,and 50-600 nm,respectively. Ellipse plane-like and long club-shaped morphology HNS were obtained using white dextrine and sodium carboxymethyl cellulose (CMC-Na) as surfactants in non-solvent,with the particle size distribution in 50-300 nm and 50-600 nm.