In order to improve the purity of RDX in nano-structured explosive by eliminating bacterial cellulose(BC),enzymatic hydrolysis was adopted. The effects of explosive on enzymatic hydrolysis of BC,such as enzyme concentration,hydrolysis time and temperature were investigated. The results show that RDX inhibits enzymatic hydrolysis of BC and hydrolysis degree of BC(HDBC) varies with enzyme concentration,hydrolysis temperature and time; HDBC increases with the increase of enzyme concentration and hydrolysis time at 45 ℃,50 ℃ and 55 ℃; the best temperature of enzymatic hydrolysis for BC is around 50 ℃.