The preparation,separation and purification of fullerene were introduced. We analyzed the preparation of fullerene by arc discharge,laser/plasma evaporation graphite method,benzene-oxygen flames synthesis,catalytic-pyrolysis of hydrocarbon,and detonation-assisted chemical vapor deposition,and considered the factors affected the preparation were the reacting region temperature,pressure,annealing temperature,catalyst,and protective gas. Comprehensive assessment of these methods shows benzene-oxygen flames synthesis has a remarkable advantage in cost,which is the mainstreaming approach in international commercial process. The difficulty and key factors lie in preparation and purification of fullerene,therefore it is important to find a quick and efficient method of separation and purification.